The expression e[(x - μ)²] represents the expected value of the squared deviation of a random variable from its mean, where 'e' denotes expectation, 'x' is the random variable, and 'μ' is the mean of that variable. This concept is crucial for understanding variance, as it quantifies how much the values of a random variable differ from their average. By analyzing these deviations, one can gain insights into the distribution and spread of the data.